Highlights
15th International Congress on Catalysis 2012
Munich/Germany
01.07.2012
- 06.07.2012
30. DECHEMA-Jahrestagung der Biotechnologen und ProcessNet-Jahrestagung 2012
Kongresszentrum, Karlsruhe
10.09.2012
- 13.09.2012
WORKSHOPS
To learn about various aspects of separation technologies four scientific workshops will be held on Sunday preceeding the symposium
Sunday, September 21, 2008 14:30 – 16:00 |
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Workshop 1
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Workshop 2 |
Sample clean up and interfacing of LC separation methods |
LC of biopolymers |
| Chair : Egidijus Machtejevas, Merck KGaA, Darmstadt/D | Chair : Friedrich Birger Anspach, HAW - Hamburg University of Applied Sciences/D |
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Sample clean up including sampling, sample treatment and prefractionation is the most decisive and essential step in biopolymer analysis. The aim is to selectively enrich the target compounds, to deplete the undesired constituents and to make the procedure robust and reproducible. A survey on the classical sample clean up methods reveals that these bear severe deficiencies. For this reason fully automated and highly selected procedures are preferred over off-line modes. Some of these methods are presented and discussed.
For the separation of proteins and peptides in biofluids multidimensional (MD) |
A basic introduction into the subject will be provided, including the most often used chromatographic modes as well as binding-elution techniques using various media. Insights will be provided on the influence of mobile phase compositions on tertiary structure. The effect of diffusion and adsorption phenomena will be addressed. |
Sunday, September 21, 2008 16:30 – 18:00 |
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| Workshop 3 | Workshop 4 |
Multidimensional LC/MS |
Basics of bioprocessing |
| Chair : Peter Schoenmakers, Polymer-Analysis Group, University of Amsterdam/NL | Chair : Alois Jungbauer, University of Natural Resources and Applied Life Sciences, Vienna/A |
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In this course the main fundamental and practical aspects of comprehensive two-dimensional liquid chromatography (LC×LC) will be discussed. The most- important question that will be addressed involves the value of LC×LC in practice. The main strengths of the technique will be discussed, viz. the possibility of generate high peak capacities and structured chromatograms. Different ways to approach (nearly) orthogonal separation systems will be discussed, including various combinations of size-exclusion chromatography, reversed-phase liquid chromatography, and ion-exchange liquid chromatography. Special attention will be paid to applications of LC×LC for the separation of proteins and peptides. The course will focus at the contemporary version of LC×LC, which typically uses two different columns and a switching valve. The common way to perform LC×LC involves a first-dimension separation that is relatively slow, with a typical analysis time of an hour or longer. The result of an LC×LC separation is a series of many (e.g. 100) fast second-dimension chromatograms. These are usually displayed as a plot that shows peak intensity as a function of the retention times in the first- and second-dimensions. In truly comprehensive separations, such a two-dimensional chromatogram is representative for the entire sample. The peak capacity of any separation can be defined as the number of peaks that can be separated with a specific resolution. The peak capacity of high- resolution one-dimensional LC is somewhere in the hundreds. Using standard commercially available columns and instruments, LC×LC allows us to achieve peak capacities in the thousands. The concept of sample dimensionality is extremely useful when sicussing LC×LC se-parations. A dimension of a sample can be seen as a property that sets the molecules apart from each other. If we manage to align the most important dimensions of the sample with the separation mechanisms we may obtain structured separations Orthogonal separations – in which the retention time in the second dimension is com-pletely independent of that in the first dimension – are ideal for LC×LC. They allow the entire “separation plane” to be filed with peaks, implying that the entire peak capacity may – in principle – be used. There are a number of ways to perform two-dimensional separations of proteins and peptides. The benchmark technique for proteins is 2D gel electrophoresis (2D-CE), which offers excellent orthogonality and a great peak capacity. However, the technique is also laborious and slow, especially when peaks need to be identified by mass spec-trometry. Potentially, LC×LC are faster and more reliable, while they also allow on-line interfacing with MS. The most common liquid- chromatographic technique for the two-dimensional separation of proteins uses ion-exchange chromatography (IEC) in first dimension and RPLC in the second. An alternative approach combines aqueous SEC with RPLC. Various opportunities and challenges faced in LC×LC will be discussed, including the types of detectors that may be used, the possibility to focus the analytes between the two separation stages, and the availability of suitable software. |
In biopharmaceutical industry chromatography is the workhorse for purification proteins, and plasmids. In this workshop an overview on the separation principles of preparative and industrial chromatography will be given. Scale up issues will be addressed in detail. The concept of constant residence time will be elaborated. Also models for scale down of chromatography will be discussed in context of spiking experiments for clearance studies of adventitious agents. Here the focus will be on the extra column peak dispersion and packing of small chromatography columns. The second part of the workshop addresses the large scale purification of plasmids, virus-like particles and viruses. Due to the restricted pore diffusion into chromatography beads other concepts of chromatographic separation have been developed. These media are based on convection. The separation principles of such media are discussed and examples are given. |
For attending the workshops registration is necessary . As the number of participants is limited, please register early to avoid disappointment.
Ticket: 50 EUR per workshop for regular participants and 25 EUR for students (only in combination with a regular conference ticket).