(subject to change)
10:30 Welcome address
10:40 BeadArray technology adds a new dimension to studies in transcriptomics, population genetics and epigenetics
F. Grädler, Illumina Inc., San Diego, CA/USA
11:00 Next generation sequencing technology enables new applications – SOLiDTM (Sequencing by Oligonucleotide Ligation and Detection)
System
T. Aspholm, Applied Biosystems Europe, Langhus/N
11:20 Next generation microarray assays: chips get hot, and chips meet enzymes
P. Stähler, C. Stähler, M. Kränzle, B. Haas, A. Thiel, H. Tronnecker, M. Beier, febit biotech gmbh, Heidelberg
11:40 Coffee break and posters
12:00 Differentiation of liquid analytes using double layer polymer films with integrated micro spot array
A. Thete, A. Gross, J.M. Köhler, TU Ilmenau
12:20 A diffusion-based microsystem for testing of cellular responses to gradients of bioactive substances
G. Roth, EMC microcollections GmbH, Tübingen; A. Ganser, Radboud University, Nijmegen/NL; D. Kern, Universität Tübingen;
K.-H. Wiesmüller, EMC microcollections GmbH, Tübingen; R. Brock, Radboud University, Nijmegen/NL
12:40 Live cell chips for isolation and characterization of stem cells from mesenchymal tissue
C. Maercker, Hochschule Mannheim; T. Rogge, Institut für Mikrostrukturtechnik, Eggenstein-Leopoldshafen; K. Bieback,
Universitätsklinikum Mannheim
13:00 Lunch
14:30 Reliable transfer of single cells to different biochip platforms
U. Müller, aura optik gmbh, Jena; M.A. Verhoff, S. Brück, Universität Gießen
14:50 DNA methylation in leukemia diagnostics: comparison of „bisulfite-deamination"- and "restriction-enzyme"-based testing
M. Hofner, C. Mandl, A. Kriegner, K. Vierlinger, R. Pichler, C. Nöhammer, A. Weinhäusel, Austrian Research Centers GmbH, Seibersdorf/A
15:10 Direct quantification of gene expression in formalin-fixed, paraffin-embedded tissues
P. Turner, Panomics Inc., Oxford/UK; W. Yang, B. Maqsodi, Y. Ma, S. Bui, K. Crawford, G. McMaster, F. Witney, Y. Luo, Panomics Inc.,
Fremont, CA/USA
15:30 Coffee break and posters
16:00 Protein lysate microarrays from formalin fixed tissue samples for nanoscale molecular diagnosis
K.F. Becker, TU München
16:20 Infrared-based protein detection arrays (iPAQ) for quantitative determination of proteins and protein modifications
C. Löbke, S.C. Schmidt, S.H. Sültmann, P.A. Poustka, K.U. Korf, DKFZ, Heidelberg
16:40 Proteome Binders: planning a European infrastructure of ligand binding molecules against the human proteome
O. Stoevesandt, The Babraham Institute, Cambridge/UK
17:00 Coffee break and posters
17:30 Plenary Lecture
A human protein atlas for normal and cancer tissues
M. Uhlen, Royal Institute of Technology, Stockholm/S
18:15 Poster Party
20:00 End of the first day
9:00 Prognostic chip for colorectal carcinomas
W. Kemmner, Max-Delbrück-Centrum, Berlin; M. Fehlker, P.M. Schlag, Charité, Berlin
9:20 Unraveling regulation mechanisms of CYP3A4 induction using gene expression profiling in human primary hepatocytes upon rifampicin
exposition
T. Reichart, S. Winter, P. Kumar, G. Vacun, Universität Stuttgart; U. Zanger, Institut für Klinische Pharmakologie, Stuttgart; V. Merk, A. Nüssler,
Humboldt-Universität Berlin; G. Redlich, E. Langenfeld, Ruhr-Universität Bochum; R. Schmid, S. Lange, Universität Stuttgart
9:40 A common gene expression signature for dilated and ischemic cardiomyopathy
R. Kuner, DKFZ, Heidelberg; A. Barth, Universitätsklinikum Großhadern, München; A. Buness, M. Ruschhaupt, DKFZ, Heidelberg; S. Merk,
Universität München; L. Zwermann, S. Kääb, E. Kreuzer, U. Mannsmann, G. Steinbeck, Universitätsklinikum Großhadern, München; A. Poustka,
DKFZ, Heidelberg; M. Näbauer, Universitätsklinikum Großhadern, München; H. Sültmann, DKFZ, Heidelberg
10:00 The Multiplex Technology in immunology: cytokines, phosphokinases and HLA/KIR typing
C. Falk, Nationales Zentrum für Tumorerkrankungen, Heidelberg; B. Simm, M. Braun, B. Mosetter, D. ter Meer, GSF Institut für Molekulare
Immunologie, München
10:20 Coffee break and posters
10:50 Plenary Lecture
Pharmacogenomics and pharmacoproteomics enabling personalized medicine for drug addiction and toxicology
S.H. Wong, Medical College of Wisconsin, Milwaukee, WI/USA
11:35 Cultivation-independent DNA microarray based approach for monitoring microorganisms in soil
C. Beckmann, G. Vogel, Kantonales Laboratorium Basel-Stadt/CH; E. Kauffmann, G. M. Kresbach, Zeptosens AG, Witterswil/CH;
U. Vögeli, Kantonales Laboratorium Basel-Stadt/CH
11:55 DNA-microarrays as a tool to investigate the genetic potential and adaptations of marine bacteria
P. Wecker, J. Peplies, A. Ellrott, F. Simonata, S. Wohlrab, F.O. Glöckner, MPI für marine Mikrobiologie, Bremen
12:15 Plenary Lecture
Making it small: protein microarrays – applications and future challenges
T.O. Joos, Natural and Medical Sciences Institute, Reutlingen
13:00 Closing remarks
13:10 Lunch and end of the Seminar
P01 Gene expression profiling of Natural Killer cell subsets isolated using novel MACS separation methods
J. Dzionek, A.M. Turkiewicz, S. Lokan, J. Schmitz, A. Bosio, V. Huppert, Miltenyi Biotec GmbH, Bergisch Gladbach
P02 New platforms and systems for DNA microarrays: 70mer oligonucleotide probes that can be used for expression analysis of splice
variants and gene families
H. Kofler, B. Saal, K. Wellesen, B. Henze, D. Drutschmann, P. Schüßler, Operon Biotechnologies GmbH, Köln
P03 Dependency of the gene regulation in glycolysis on the glucose concentration
B. Grote, C. Klockow, F. Stahl, B. Hitzmann, Universität Hannover
P04 Induction of stress genes of Saccharomyces cerevisiae during starvation
C. Klockow, F. Stahl, B. Hitzmann, Universität Hannover
P05 Molecular signatures from highly parallelized platforms can serve as multiplex biomarkers and help elucidate the complexity of biological
systems
M. Hirt, M. Vanetti, IMGM Laboratories GmbH, Martinsried
P06 Anti-inflammatory drugs and pancreatic carcinoma
M. Youns, A. Bauer, J. Hoheisel, T. Efferth, DKFZ, Heidelberg; J. Reichling, Universität Heidelberg
P07 Determination of the autoantibody repertoire in autoimmune diseases with protein arrays - a new approach to personalized therapies
A. Lüking, C. Gutjahr, K. Schulte, V. Trappe, M. Ciupke, Protagen AG, Dortmund; T. Niehues, Universitätsklinikum Düsseldorf;
S. Schimrigk, Klinikum der Ruhr-Universität Bochum; M. Schneider, Universitätsklinikum Düsseldorf; H.E. Meyer, Medical Proteom Center,
Ruhr-Universität Bochum; S. Müllner, J. Beator, Protagen AG, Dortmund
P08 Transcriptional analysis of the protein secretion pathways sec and TAT in E. coli
F. Stahl, M. Reck, C. Repenning, B. Hitzmann, T. Scheper, Universität Hannover; K. Friehs, B. Sommer, F. Aster, E. Flaschel, Universität Bielefeld
P09 Entwicklung von PNANA-Chips zur spezifischen Identifizierung illegaler Öl- und Treibstoffeinleitungen in die Weltmeere
A. Nies, A. Jacob, J. Hoheisel, DKFZ, Heidelberg; N. Marmé, Universität Heidelberg; J.-P. Knemeyer, DKFZ, Heidelberg
P10 Convenient and robust one-step cDNA labeling for direct microarray hybridization
C. Reinhard, A. Bosio, V. Nölle, Miltenyi Biotec GmbH, Bergisch Gladbach
P11 Development of a panel of DNA microarray performance indicators
T. Wilkes, H. Laux, C. Foy, LGC Ltd, Teddington/UK
P12 A bi-functional MIP: analysis of binding and catalytical activity by a thermistor
M. Katterle, K. Lettau, A. Warsinke, F.W. Scheller, Universität Potsdam
P13 Entwicklung von aptamerbasierten Protein Microarrays für biotechnologische Anwendungen
J.-G. Walter, F. Stahl, Universität Hannover; J. Glöckler, RiNA GmbH, Berlin; T. Scheper, Universität Hannover
P14 Development of quantitative protein detection arrays
F. Henjes, DKFZ, Heidelberg; A. Tresch, Universität Mainz; S. Derdak, T. Beissbarth, U. Klingmüller, A. Poustka, U. Korf, DKFZ, Heidelberg
P15 Unravel the ER-alpha pathway in breast cancer: a combinatorial approach of RNA interference and DNA microarray technology
M. Fellmann, A. Buness, T. Beissbarth, M. Ruschhaupt, DKFZ, Heidelberg; A. Tresch, Universitätsklinikum Mainz; R. Kuner,
A. Poustka, H. Sültmann, DKFZ, Heidelberg
P16 Neue Auswerteverfahren zur robusten Quantifizierung von Mikroarray-Daten
C. Repenning, F. Stahl, Universität Hannover; K. Fries, Universität Bielefeld; T. Scheper, B. Hitzmann, Universität Hannover
P17 Advanced discovery of protein biomarkers by serum prefractionation
R. Stempfer, Austrian Research Centers GmbH, Seibersdorf/A; C. Gerner, Medical University, Vienna/A; C. Reichel, C. Nöhammer, Austrian
Research Centers GmbH, Seibersdorf/A
P18 Effects of CLU-502 on pancreatic cancer cells analyzed by gene expression profiling
F. Holtrup, A. Bauer, DKFZ, Heidelberg; R.A. Hilger, Westdeutsches Tumorzentrum, Essen; J.D. Hoheisel, DKFZ, Heidelberg
P19 Microfluidic platform for parallel processing of microarrays
C. Mittermayr, P. Winklehner, F. Winner, Lambda GmbH, Freistadt/A; N. Gottschlich, J. Stappert, Greiner Bio-One GmbH, Frickenhausen
P20 Comparison of long oligonucleotide versus short oligonucleotide microarray platforms for ChIP-chip experiments
F. Wagner, RZPD Deutsches Ressourcenzentrum für Genomforschung, Berlin; D. Schmidt, MPI für molekulare Genetik, Berlin;
K. Jürchott, S. Hennig, RZPD Deutsches Ressourcenzentrum für Genomforschung, Berlin; I. Piccini, M.-L. Yaspo, MPI für molekulare Genetik,
Berlin; M. Schad, RZPD Deutsches Ressourcenzentrum für Genomforschung, Berlin
P21 Protein microarrays for the quantification of microorganisms in drinking water
A. Wolter, M. Seidel, R. Nießner, TU München
P22 A database system for managing of clone libraries, protein expressions and protein biochip content
K. Marquart, C. Scheer, V. Trappe, K. Schulte, J. Beator, A. Lüking, M. Blüggel, Protagen AG, Dortmund; R. Thiele, Bonn-Rhein-Sieg University
of Applied Science, St. Augustin
P23 Development of a regenerable immunosensor array for the rapid parallel detection of antibiotics in milk
K. Kloth, A. Wolter, M. Seidel, R. Nießner, TU München; A. Didier, R. Dietrich, E. Märtlbauer, Universität München; M.G. Weller, BAM, Berlin
P24 A splicing-sensitive microarray strategy for profiling alternative splicing patterns from functional genes and corresponding pseudogenes
M.H. Hofmann, E. Schaeffeler, K. Klein, M. Eichelbaum, M. Schwab, Dr. Margarete Fischer-Bosch-Institut, Stuttgart; T.T. Bachmann, Universität
Stuttgart; U.M. Zanger, Dr. Margarete Fischer-Bosch-Institut, Stuttgart
P25 The MiCoNet "Chip Design Internet Service" (CDIS) and an application to the biodiversity EU project "Fish & Chips"
M. Nölte, H. Weber, Universität Bremen
P26 First steps towards multiprotein analysis of colon cancer progression using a NexterionŇ Slide H
M. Radeva, Leibniz Institute for Age Research, Jena; D. Knoll, SCHOTT Jenaer Glas GmbH, Jena; T. Hofmann, Universität Jena; C. Schwung,
SCHOTT Jenaer Glas GmbH, Jena; J. Sauer, Universität Jena; K.O. Greulich, Leibniz Institute for Age Research, Jena; B. Pool-Zobel, Universität
Jena
P27 Usage of an archive of standardized DNA microarray experiments for visualization and meta-analysis of co-regulated inflammatory genes
P28 Identification of mediators in hyper-inflammatory diseases using miniaturized and parallelized sandwich immunoassays
H. Hsu, NMI Reutlingen; M. Weiss, F. Kriebel, E.M. Schneider, Universitätsklinikum Ulm; M.F. Templin, T.O. Joos, NMI Reutlingen
P29 Antibody microarrays based on microstructured surface-attached hydrogels for amplified signals
M. Moschallski, J. Rühe, Universität Freiburg
P30 Microarray read-out technology using attenuated total reflection
T. Brefka, T. Neumann, T. Brandstetter, J. Rühe, Universität Freiburg
P31 Ein HPV (Human Papilloma Virus) Typisierungskonzept basierend auf Mikroarray-Technologie
K. Wagner, Universität Freiburg; J. Alt-Mörbe, Labor für DNA-Analytik, Freiburg; T. Brandstetter, J. Rühe, Universität Freiburg
P32 Designing customized CpG Island arrays by the novel Gene Filter Software
A. Kriegner, I. Visne, A. Yildiz, A. Weinhäusel, K. Vierlinger, C. Nöhammer, Austrian Research Centers GmbH, Seibersdorf/A
P33 Characterisation of myoglobin-deficient mouse model using oligonucleotide-based microarrays
A. Molojavyi, A. Lindecke, A. Gödecke, K. Köhrer, Universität Düsseldorf
P34 The SELDI-based ProteinChip® System in clinical, process and research proteomics
A. Wiesner, Bio-Rad Laboratories GmbH, München
P35 Employment of the immune toxicity chip for in vitro toxicological testing
S. Szameit, K. Vierlinger, M. Mansfeld, H. Tuschl, C. Nöhammer, Austrian Research Centers GmbH, Seibersdorf/A
P36 Expression analysis of the marine polymer degrader Gramella forsetii strain KT0803
F. Simonato, M. Bauer, P. Wecker, M. Richter, R. Amann, F.O. Glöckner, MPI für marine Mikrobiologie, Bremen
P37 Human pathogen detection using DNA chips and identification by pattern recognition
H. Wiesinger-Mayr, R. Pichler, K. Vierlinger, L. Bodrossy, Austrian Research Centers GmbH, Seibersdorf/A; A.M. Hirschl, E. Presterl, Medical
University, Vienna/A; C. Nöhammer, Austrian Research Centers GmbH, Seibersdorf/A
P38 Parallel synthesis and purification for the production of peptide arrays
M. Dauber, A. Stephan, M. Schnölzer, J.D. Hoheisel, A. Jacob, DKFZ, Heidelberg
P39 Matrix-CGH zur hochauflösenden genetischen Analyse der Nierenzellkarzinome
J. Sanjmyatav, Universität Jena; S. Wessendorf, C. Schwaenen, Universitätsklinikum Ulm; K. Junker, Universität Jena
P40 A novel biochip platform focussing on clinical requirements
T. Brandstetter, K. Wagner, T. Brefka, T. Neumann, M. Moschallski, J. Rühe, Universität Freiburg
P41 Visualization of genomic aberrations using Affymetrix SNP arrays
A. Müller, Universitätsklinikum Ulm; K. Holzmann, Universität Ulm; H.A. Kestler, Universitätsklinikum und Universität Ulm
P42 New applications for microarray technologies
H. Schröder, Universität Dortmund; B. Ellinger, H. Waldmann, MPI für molekulare Physiologie, Dortmund; C. Niemeyer, M. Fleger, A. Neyer,
Universität Dortmund
P43 An empirical comparison of feature reduction methods in the context of microarray data classification
H.A. Kestler, C. Müssel, Universität Ulm; M. Buchholz, T.M. Gress, Universitätsklinikum Marburg
P44 A perceptually optimized scheme for visualizing gene expression ratios with confidence values
H.A. Kestler, A. Müller, Universität Ulm; M. Buchholz, T.M. Gress, Universitätsklinikum Marburg
P45 Identification of serum amyloid alpha by proteome analysis as a specific biomarker in serum of patients with renal cell carcinomas
R. Pilchowski, J. Müller, F. von Eggeling, J. Schubert, K. Junker, Universitätsklinikum Jena
P46 Genotyping DNA microarray for the simultaneous detection of pathoadaptive mutations and quinolone resistance in Escherichia coli
T. Barl, X. Yu, Universität Stuttgart; U. Dobrindt, J. Hacker, Universität Würzburg; T. Bachmann, Universität Stuttgart
P47 Analysis of gene expression in Trypanosoma brucei
R.A. Queiroz, DKFZ, Heidelberg; C.E. Clayton, Universität Heidelberg; J.D. Hoheisel, DKFZ, Heidelberg
P48 New synthesized ferrocene-redox polymer for the electrochemical detection of soluble glucose dehydrogenase
B. Nagel, M. Katterle, F.W. Scheller, U. Wollenberger, A. Warsinke, Universität Potsdam
P49 Expanding assay dynamics: a combination of competitive and direct immunoassays for the quantification of proteins in microarray based
systems
M. Hartmann, A. Döttinger, NMI Reutlingen; J. Roeraade, KTH, Stockholm/S; T.O. Joos, M.F. Templin, NMI Reutlingen
P50 Protein microarray analysis by fluorescence lifetime imaging
S. Peters, Institute for Physical High Technology, Jena; R. Dietrich, Schott Jenaer Glas GmbH, Jena; K.O. Greulich, P. Grigaravicius, Leibniz-
Institute for Age Research, Jena; F. Liebold, Institute for Physical High Technology, Jena; D. Knoll, Schott Jenaer Glas GmbH, Jena;
P. Schellenberg, Institute for Physical High Technology, Jena
P51 Properties of fluorescence dyes used for labeling DNA in microarray experiments
J. Sobek, Functional Genomics Center, Zürich/CH
P52 DNA-chip-based determination of binding specificities of transcription factors (Tfs) linked to colorectal cancer
C. Krauß, N. Tonisson, DKFZ, Heidelberg; J. Taipale, Universität Helsinki/FIN; J.D. Hoheisel, DKFZ, Heidelberg
P53 Bringing DNA-microarrays to the field: on-site identification and toxicity assessment of cyanobacteria at Lake Baikal in Siberia
H. Peter, S.-H. Sun, Universität Stuttgart; I. Tikhonova, A. Gladkikh, O.I. Belykh, V. Parfenova, Limnological Institute of SB RAS, Irkutsk/RUS;
T.T. Bachmann, Universität Stuttgart
P54 Developing oligonucleotide arrays for quantitative analysis of methylation patterns of tumor suppressor genes in breast cancer
Y. Riazalhosseini, V. Beier, J.D. Hoheisel, DKFZ, Heidelberg
P55 Analysis of signal transduction networks involved in the adhesion of encephalitogenic lymphocytes by using high-throughput technology
L. Ottoboni, M. Frohme, DKFZ, Heidelberg; B. Rossi, S. Bach, University of Verona/I; K. Fellenberg, DKFZ, Heidelberg;
G. Constantin, University of Verona/I; J.D. Hoheisel, DKFZ, Heidelberg
P56 Peptide-based sandwich immunoassays: a novel approach to quantify phospho-proteins
O. Pötz, NMI Reutlingen; T. Nadler, J. Boucher, N. Gordon, Epitome Biosystems, Waltham, MA/USA; S. Pabst, M.F. Templin, D. Stoll,
T.O. Joos, NMI Reutlingen
P57 Antibody microarrays: towards a highly multiplex plasma protein profiling
C. Schröder, P. Angenendt, J. Linseisen, W. Kusnezow, A. Nieters, K. Fellenberg, J.D. Hoheisel, DKFZ, Heidelberg
P58 Diagnostic microarray assay for SNP-typing of the gene encoding the drug metabolising enzyme arylamine n-acetyltransferase 2 (NAT2)
T. Pfeiffer, EUROIMMUN Medizinische Labordiagnostika AG, Lübeck; R. Gruber, W. Kuon, Medizinische Universitätsklinik München;
H. Plischke, Universität München; S. Kirsch, Medizinische Universitätsklinik München; D. Körner, S. Zieseniss, EUROIMMUN Medizinische
Labordiagnostika AG, Lübeck; M. Schattenkirchner, Privatpraxis, München; W. Stoecker, U. Steller, EUROIMMUN Medizinische
Labordiagnostika AG, Lübeck
P59 The Hydractina echinata EST and functional genome analysis project
J. Soza-Ried, J.D. Hoheisel, DKFZ, Heidelberg; U. Frank, National University of Ireland, Galway/IRL; M. Frohme, DKFZ, Heidelberg
P60 Decyphering the antibodyOME - peptide chips for routine high throughput seroanalytics
C. Grötzinger, Charité, Berlin; H. Andresen, FhI für Biomedizinische Technik, Potsdam; O.J. Kreuzer, peptides&elephants GmbH, Potsdam;
J. M. Hollidt, in.vent Diagnostica GmbH, Hennigsdorf; F.F. Bier, FhI für Biomedizinische Technik, Potsdam
P61 Infrared versus visible fluorophores for detection of protein and DNA arrays
O. Menssing, LI-COR Biosciences, Bad Homburg